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- 英文名:
yuanmu
- 库存:
100
- 供应商:
上海远慕生物有限公司
品牌:Omega
货号:PS010
产品名称:BAC Binding Buffer,250ml
质粒相关溶液
BAC Binding Buffer,250ml(Omega 耗材与配件、溶液) 上海远慕生物有限公司专业供应各种生物试剂、生化试剂,单抗多抗抗体,各种二抗,标记抗体,抗体抗原,蛋白多肽产品,Elisa酶联免疫试剂盒,各种细胞株、细胞系,各种动物血清,金标试剂盒,放免试剂盒,培养基,毒素 的产品, 实时价格和产品详细说明,敬请来电咨询!
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文献和实验Isolation of CpG Islands From BAC Clones Using a Methyl-CpG Binding Column
Vertebrate genomes are globally heavily methylated at the sequence CpG with the exception of short patches of GC-rich DNA, usually between 1–2 kb in size, which are free of methylation and these are known as CpG islands (see refs
.Before Beginning:Prepare a 20 h culture of BAC containing bacteria in 2X YT and appropriate antibiotic. The OD600 of the final culture should be 5.0 -0.5.Add RNAse A to E1 to a final concentration of 400 µg/mL.Increase NaCl concentration in Wash Buffer (E5
Preparation of BAC (Bacterial Artificial Chromosome) DNA with by Alkaline Prep Method 1. Grow 250 ml of LB culture at 37 °C for 16 hours (> 250 rpm) with the appropriate antibiotic. Cultures can be started by 1) picking a single colony
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