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phospho-MLC/Myosin light chain

(Ser20) Antibody
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  • ¥2400
  • PLLABS已认证
  • 加拿大
  • PL0301021
  • 2025年07月12日
  • WB: 1:100-500 E: 1:500-1000 IP: 1:20-100 IHC: 1:100-500 IF: 1:100-500
  • Rabbit
  • Hu, Mo, Rat, Dog, Pig, Cow; Sheep,
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      PhosphoPeptide derived from Hu Myosin light chain around the phosphorylation site of Ser20.

    • 亚型

      Rabbit Polyclonal IgG

    • 形态

      液态

    • 保存条件

      保存于-20℃

    • 克隆性

      多克隆

    • 标记物

      无标记(PLlabs可供应生物素、酶、荧光素标记的抗体)

    • 适应物种

      Hu, Mo, Rat, Dog, Pig, Cow; Sheep,

    • 保质期

      按说明书条件可保存1-2年

    • 抗原来源

      见详细说明资料

    • 目录编号

      PL0301021

    • 级别

      超纯

    • 库存

      大量

    • 供应商

      华拓生物

    • 宿主

      Rabbit

    • 应用范围

      WB: 1:100-500 E: 1:500-1000 IP: 1:20-100 IHC: 1:100-500 IF: 1:100-500

    • 浓度

      1mg/ml

    • 靶点

      phospho-MLC/Myosin light chain(Ser20)

    • 抗体英文名

      phospho-MLC/Myosin light chain(Ser20) Antibody

    • 抗体名

      磷酸化肌球蛋白轻链激酶抗体

    • 规格

      0.1ml

    产品细节图片1
    phospho-MLC/Myosin light chain(Ser20) Antibody
    Cat#:PL0301021
    Isotype:Rabbit Polyclonal IgG
    Immunogen:PhosphoPeptide derived from Hu Myosin light chain around the phosphorylation site of Ser20.
    Purification: Immunogen affinity purified

    Storage Buffer: PBS, pH 7.4(1%BSA and 0.1% Sodium azide)

    Background:
    Myosin light chain (MLC) is a subunit of the conventional myosins (e.g. myosin II). In smooth muscle and non-muscle cells conventional myosins mediate a wide variety of contractile events including cytokinesis, cell motility, and smooth muscle contraction. MLC is phosphorylated by multiple serine-threonine kinases such as Rho-kinase and PAK, however myosin light chain kinase (MLCK) acts as the primary kinase. Contractile activity of conventional myosins is regulated by phosphorylation of MLC on several residues.
    Alternate Names:
    p-MLC(Ser20); phospho-MLC(Ser20); p-Myosin light chain(Ser20); Myosin light chain(Ser20); A1 catalytic; A2 catalytic; Alkali myosin light chain 1; Alkali myosin light chain 3; C36E6.3; C36E6.3; CMH10; DKFZp779C0562; KMLC; MLC 2; MLC 2v; MLC; MLC1F; MLC2; MLC3F; MLCK2; MRLC1; MYL 1; MYL 2; MYL1; MYL1 protein; MYL2; MYL9; MYLK2; Myosin light chain 1 alkali skeletal fast; Myosin light chain 1 skeletal muscle isoform; Myosin light chain 2 regulatory cardiac slow; Myosin light chain 3 skeletal muscle isoform; Myosin light chain kinase 2; Myosin regulatory light chain 2 ventricular/cardiac muscle isoform; Myosin regulatory light chain 2, smooth muscle isoform; Myosin, light chain 9, regulatory; MYRL2; Skeletal myosin light chain kinase.
    Specificity Reaction:Hu, Mo, Rat, Dog, Pig, Cow; Sheep,
    Application Notes:WB: 1:100-500 E: 1:500-1000 IP: 1:20-100 IHC: 1:100-500 IF: 1:100-500
    Storage :
    Store at –20ºC for one year. Avoid repeated freeze-thaw cycles. At 4ºC for 2-3 weeks after reconstituted.

    This product for research use only, not for use in humans, therapeutic or diagnostic procedures.
    PLlabs公司可供应相应抗体的生物素、辣根过氧化物酶、荧光素(FITC、PE、 CY3、 CY5、 CY7、 PE- CY3/5/7)标记抗体,欢迎大家咨询订购。

    产品细节图片2 产品细节图片3 产品细节图片4
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    图标文献和实验
    相关实验
    • Myosin Light Chain Preparation

      in lyophilizer bottles and freeze in liquid nitrogen.Put on lyophilizer and leave until dry (overnight or over weekend). Day 5 22.Resuspend separate light chain pools 1 and 2 in a small volume of Dialyzing Solution G.Pipette into dialyzing tubing (size #20

    • Purification of Myosin Light Chain Kinase

      RPM (4℃).Filter the supernatant through glass wool and discard the pellet (or save for light chain prep).Save 1 ml of supernatant (4℃)for assay.Add pepstatin and TLCK to final concentrations of 1 μg/ml and 40 Og/ml,respectively. Volume of supernatant

    • Structure and Properties of Human Immunoglobulin Light-Chain Dimers

      Antibodies are multisubunit proteins, but unlike many other multisubunit proteins, it is possible to study structural and functional properties of an antibody subunit free of the others. Thus, antibodies are singular proteins

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