- ￥1700 - 4000
- GeneTex
- 美国
- GTX100714
- 2025年07月16日
- WB, IHC-P
- Rabbit
- Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Myosin Light Chain 2 (MLC-2v). The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Mouse, Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX100714
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, IHC-P
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
This antibody detects Myosin light chain 2, slow skeletal/ventricular muscle isoform (MLC-2v) protein.
- 抗体英文名：
Myosin Light Chain 2 (MLC-2v) antibody
- 抗体名：
Myosin Light Chain 2 (MLC-2v) 抗体
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Myosin Light Chain 2 (MLC-2v) antibody detects Myosin Light Chain 2 (MLC-2v) protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
Myosin Light Chain 2 (MLC-2v) stained by Myosin Light Chain 2 (MLC-2v) antibody (GTX100714) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Myosin Light Chain 2 (MLC-2v) antibody detects Myosin Light Chain 2 (MLC-2v) protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat heart.
Myosin Light Chain 2 (MLC-2v) stained by Myosin Light Chain 2 (MLC-2v) antibody (GTX100714) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Whole cell extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Myosin Light Chain 2 (MLC-2v) antibody (GTX100714) diluted at 1:30000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Myosin Light Chain 2 antibody (GTX100714) diluted at 1:20000.
Myosin Light Chain 2 (MLC-2v) antibody detects Myosin Light Chain 2 (MLC-2v) protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
Myosin Light Chain 2 (MLC-2v) stained by Myosin Light Chain 2 (MLC-2v) antibody (GTX100714) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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文献和实验Myosin Light Chain Preparation
in lyophilizer bottles and freeze in liquid nitrogen.Put on lyophilizer and leave until dry (overnight or over weekend). Day 5 22.Resuspend separate light chain pools 1 and 2 in a small volume of Dialyzing Solution G.Pipette into dialyzing tubing (size #20
Purification of Myosin Light Chain Kinase
RPM (4℃).Filter the supernatant through glass wool and discard the pellet (or save for light chain prep).Save 1 ml of supernatant (4℃)for assay.Add pepstatin and TLCK to final concentrations of 1 μg/ml and 40 Og/ml,respectively. Volume of supernatant
Labeling Gizzard Myosin with Tetramethylrhodamine Iodoacetamide (Primarily 17kDa Light Chain)
Day 1 Materials 1.50 mM KCl,0.2 mM EGTA,2 mM ATP,2 mM MgCl2,10 mM HEPES,pH 7.0.Need 500 ml. Procedure 1.Thaw appropriate volume of frozen gizzard myosin to obtain 10-20 mg. 2.Centrifuge in a SS34 rotor at 9,000 rpm,4℃ for 20 min. 3.Resuspend
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