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西宝生物
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文献和实验ELECTROPORATION OF ES CELLS AND ISOLATION OF H/R CLONES
4 2 x 106 EP + DNA Normal % survival after EP + DNA 5 2 x 106 EP + DNA G418 % total integrants ie random + H/R's
ELECTROPORATION OF ES CELLS AND ISOLATION OF H/R CLONES
5 2 x 106 EP + DNA G418 % total integrants ie random + H/R's 6 2 x 106 EP + DNA G418* Same as Plate 5 unless 7 2 x 106
A quick RNA mini-prep for Neurospora mycelial cultures
water bath, occasionally shaking, to get SDS into solution. This should take about 5 minutes. 6. Add 1.1 ml of 3M KCl, invert to mix, keep on ice for 10 min. Solution should form semi-solid, flocculent mass as K-SDS precipitate forms. 7. Spin
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