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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
STD. GRAD BLUE TIP, 101-1000 µL RACKED, STERILE
- 供应商：
西宝生物
- 库存：
1 each
- 保存条件：
ROOM TEMPERATURE
- 规格：
LABWARE
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文献和实验Transformation of Electrocompetent E. coli with Blue/White selection
Desalt DNA template by EtOH precipitation in NaOAc followed by at least 2x washes with 70% EtOH. Resuspend in 5 - 15 µL of sterile H2 O. Rinse cuvettes (if they have been used before) 5x with deionied H2 O, and place them on ice
blade. Place the piece of gel/paper in a microfuge tube. Add 100 µl water. After 2-5 min. peel the acrylamide off the paper. Place the acrylamide in a new tube. Grind the acrylamide with a pipet tip. Add 100 µl water.
Clonality - X Chromosome Inactivation Assay
for 5 min at 94°C. Load 3 µl onto gel. Electrophorese at 1800 volts for 1 to 2 hours. TIP: In clonality analysis, the PCR products are ~200 bp. Therefore do not overload the gel with sample and run the gel for a longer period of time to sharpen
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