Histone H3 (K9/14)乙酰化多克隆抗体(50

浅析染色质免疫沉淀（ChIP）技术在 DNA 与蛋白质相互作用研究中的重要性

因子的结合位置。 ChIP 是染色质结构改变，特别是组蛋白修饰后，研究全基因组活化或抑制作用的少数工具之一。ChIP 可以针对乙酰化或者甲基化的组蛋白，富集该区域的 DNA ，因此可以进行基因组的多样性分析。2008 年，Xiang 等人 [7] 发现硒（Se）处理与前列腺癌细胞的 DNA 甲基化水平降低、组蛋白乙酰化水平升高、GSTP1（前列腺癌相关蛋白）活化有关。试验中，他们用 ChIP 技术富集了乙酰化 H3-k9 和甲基化 H3-K9，对分离获得的 DNA 进行多个基因的 PCR 扩增

RNA-biotin based pulldown assays for the detection of siRNA targeted genomic regions and siRNA directed histone modifications (PROT32)

chromatin while keeping samples on ice (1 interval for 20 seconds at power setting of "3" on the Branson 50 cell machine). Immediately centrifuge at 14,000 RPM for 10 minutes at 4°C;Carefully remove the supernatant and transfer to a new tube. Preclear

Competing Chromosomal Proteins from Drosophila Polytene Chromosomes Using Modified Histone Peptides

to chromatin, by its histone tail interaction or by other interactions, then you would not expect to see loss of binding upon peptide competition. A good positive control for the assay is to use H3K9 dimethylated peptide to compete HP1 from the pericentric