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- 英文名:
yuanmu
- 库存:
100
- 供应商:
上海远慕生物有限公司
产品名称:Endo-free Plasmid Mini Kit I(50)(Omega 质粒抽提试剂盒)
品牌:Omega
产品货号:D6948-01无内毒素小量质粒提取试剂盒 I/II:从培养过夜的菌液中提取30ug/70ug无内毒素质粒DNA
Endo-free Plasmid Mini Kit I(50)(Omega 质粒抽提试剂盒)上海远慕生物有限公司专业供应各种生物试剂、生化试剂,单抗多抗抗体,各种二抗,标记抗体,抗体抗原,蛋白多肽产品,Elisa酶联免疫试剂盒,各种细胞株、细胞系,各种动物血清,金标试剂盒,放免试剂盒,培养基,毒素 的产品, 实时价格和产品详细说明,敬请来电咨询!
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Endo-free Plasmid Mini Kit I(50)(Omega 质粒抽提试剂盒)
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文献和实验E.Z.N.A.® Endo-free Plasmid Mini Kit I Spin Protocol
实验步骤 1. Inoculate 5ml LB/ampicillin (50 ug/ml) medium placed in a 10-20 ml culture tube with E.coli carrying desired plasmid and grow at 37°C with agitation for 12-16 h. It is strongly recommended that an endA negative
E.Z.N.A. Endo-Free Plasmid Mini Kit II Spin Protocol
ethanol (room temperature, 96-100%). Gently mix by inverting tube 6-7 times and incubate at room temperature for1-2 minutes. Plasmid DNA Purification with the HiBind® DNA Mini Column II: 8. Equilibrate the HiBind® DNA column: Add 100ul
Plasmid Mini Purification Protocol
before doing plasmid prep: •Put 4 ml of LB (with appropriate antibiotic added) into a 50-ml conical bottom tube.Add one colony of the desired bacterial/plasmid culture and incubate at 37° with shaking overnight.It’s essential to use selective media
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