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- 供应商:
上海远慕生物有限公司
产品名称: Plasmid Mini Kit II(200)(Omega 质粒抽提试剂盒)
品牌:Omega
产品货号:D6945-02
质粒小量提取试剂盒 II型 :从小于15ml培养过夜的菌液提取50-70ug高纯度质粒DNA
Plasmid Mini Kit II(200)(Omega 质粒抽提试剂盒) 上海远慕生物科技有限公司提供多种生化试剂,经营产品包括各种种属的ELISA试剂盒,高纯度的生化试剂、分析试剂、化工中间体、催化剂、溶剂等各类化工产品,品种达数万种等等。公司确保每个产品的质量,价格合理,为客户提供满意的产品,请您放心购买!
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文献和实验E.Z.N.A. Endo-Free Plasmid Mini Kit II Spin Protocol
ethanol (room temperature, 96-100%). Gently mix by inverting tube 6-7 times and incubate at room temperature for1-2 minutes. Plasmid DNA Purification with the HiBind® DNA Mini Column II: 8. Equilibrate the HiBind® DNA column: Add 100ul
E.Z.N.A.® Endo-free Plasmid Mini Kit I Spin Protocol
gently but throughly by inverting and rotating tube 4-6 times to obtain a cleared lysate. A 2 min incubation at room temperature may be necessary. Avoid vigorous mixing as this will shear chromosomal DNA and lower plasmid purity. (Store Solution II
1. Spin down 1.5 ml of overnight culture in 2ml or 1.5ml tube for 1 minute on high. 2. Asprirate supernatant and resuspend cell pellet in 100 µl Solution I (25 mM Tris-HCl, pH 8.0, 10 mM EDTA). 3. Add 200 µl Solution II (0.2 N NaOH, 1.0% SDS
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