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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
艾美捷科技有限公司
- 规格:
2μg&10μg&1mg
【产品形式】:艾美捷为您提供的【sCD4】重组人可溶性CD4蛋白产品是是在杆状病毒-昆虫Sf9细胞中重组表达的,是一条单链、糖基化的多肽链,包含有365个氨基酸。非糖基化蛋白分子量为45kDa,由于发生了糖基化修饰,其在SDS-PAGE电泳显示分子量为46kDa。该人可溶性CD4重组蛋白最终由标准的色谱技术纯化出。
【背景资料】:CD4 is a cell-surface glycoprotein found on the mature helper T cells and immature thymocytes,as well as on monocytes and macrophages. (Some cytotoxic T cells have CD4 protein as well.) Normally,about 65% of T cells in the blood are CD4+ (have CD4 protein protruding from their membrane). A mature T cell with either have CD4 or CD8,but not both. During one stage of development T cells develop CD4 and CD8 receptors,but they eventually are differentiated in the thymus and become more specialized.
以色列ProSpec-Tany公司专注于细菌来源重组蛋白质生产。其独有细菌和哺乳动物表达和蛋白折叠技术使其成为国际一流科研级蛋白供应商。ProSpec-Tany是世界上能提供蛋白种类最多公司之一。领先生产工艺和规模使其可以提供毫克到克级蛋白,价格优于同类公司。作为多家知名品牌代理,艾美捷与ProSpec一起为研究者们提供最好、最新细胞生物学研究工具而不断扩大产品及服务质量。更多特色试剂盒、特色抗体以及特色试剂等产品评论,请点击查看中国生命科学试剂产品评论网站。
点击【sCD4】重组人可溶性CD4查看更详细产品说明,更多应用、储存、价格、货期等信息请致电400-6800-1454垂询Prospec中国区域授权总代理艾美捷科技有限公司。
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文献和实验Tracking Antigen Specific CD4+ T-Cells With Soluble MHC Molecules
The advent of soluble MHC multimer technology has allowed for the flow-cytometric direct identification of specific-MHC restricted antigen-specific T cells in mixed cell populations and also enabled the direct phenotyping and cloning
Many cytokines alter the half-lives and stabilities of mRNAs for other cytokines (1 ) and enzymes that may be related to disease progression. For example, IL-1β stabilizes the mRNA for human interstitial collagenase-1 in normal fibroblasts
Production and Characterization of Recombinant Human and Murine TNF
Here we describe the methods for the expression of human and murine soluble tumor necrosis factor (hTNF and mTNF) in Escherichia coli cells and for their extraction, purification, and characterization. The expression and purification procedure
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