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![RAD17 Monoclonal Antibody [2G12] (100 ug), RAD17单克隆抗体[2G12] (100 ug)](https://custom.dxycdn.com/trademd/upload/pic/2009/09/30/1254281266.gif!small.t.1)
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文献和实验Chick Chorioallantoic Membrane (CAM) Assay
for gross evaluation. For injection, borosilicate glass capillaries (OD 1.0 mm, ID 0.75 mm; Sutter Instrument Company, Novato, CA) were prepared with P-87 micropipette puller (Sutter Instrument Company). Needles were connected with Tygon tubing (ID 1/32
Fluorescein labeling of proteins
as preservative. Determine concentration and F/P ratio by measuring absorbance at 280 nm and at 495 nm.For antibodies,use the following formulae to get approximate values: Protein concentration (mg/ml)= (OD280 - 0.35 x OD495)/ 1.4 F/P ratio = (3.3 x OD495)/ (OD
Fluorescein labeling of proteins
. Determine concentration and F/P ratio by measuring absorbance at 280 nm and at 495 nm. For antibodies , use the following formulae to get approximate values: Protein concentration (mg/ml) = (OD280 - 0.35 x OD495 ) / 1.4 F/P ratio = (3.3 x OD495 ) / (OD
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