HLA-ABC, CommonMONOCLONAL ANTI

The Avidin-Biotin Complex(ABC) Method

, between the horseradish peroxidase enzyme and a secondary antibody reagent, enzyme localization can be achieved at the site of primary antibody interaction with the specimen (see ) Fig. 1 ). The biotin molecule is small and can be easily conjugated to immunoglobulin

AbC Anti-Mouse Bead Kit

a sample tube for each fluorochrome-conjugated mouse antibody you are using, and add 1 drop of AbC™ capture beads (Component A) to each tube. 3)        Add a pre-titrated amount of each mouse antibody conjugate to the AbC™ capture bead suspension

AbC Anti-Rat/Hamster Bead Kit

capture beads (Component A) and negative beads (Component B) by gently vortexing for 30 seconds before use. 2)      Label a sample tube for each fluorochrome-conjugated rat or hamster antibody you are using, and add 1 drop of AbC™ RH capture beads