【产品形式】:艾美捷为您提供的泛素蛋白-Lys-TAMRA形式为Lyophilised. Dissolve in DMSO to a final concentration of 100 μM (0.916 mg/mL) before diluting slowly into required aqueous buffer
【背景资料】:Fluorescence polarisation assay reagent based on a 5-carboxytetramethylrhodamine (TAMRA) modified Lys-Gly sequence linked to ubiquitin via a native isopeptide bond with the lysine side-chain. Has utility as a deubiquitinating enzyme (DUB) substrate for high sensitivity continuous output at longer wavelengths than traditional fluorophores helping to overcome problems of compound auto-fluorescence or quenching in screening assays. Cleavage of isopeptide bond more closely represents the 'natural' action of some DUBs and results in a decrease in fluorescence polarisation output.