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PHOSPHODIESTERASE II
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件:
0 C
- 供应商:
西宝生物
- 英文名:
CYTOCHALASIN A
- 库存:
1 mg
- 规格:
BIOCHEMICALS
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文献和实验C29 H37 NO3 。从真菌类 Helminthosporium de- mariodeum中分离得到的代谢产物。是以希腊语的 cytos(细胞)和 chalasis(松弛)组合而命名的。在许多动植物细胞的细胞运动中,特别是可逆地抑制同微丝系有关的现象。例如在 0.5— 1.0微克/毫升浓度下作用 24小时,会使鼠的培养纤维芽细胞只进行核分裂,而细胞质的分裂受到抑制,从而成为多核细胞。 5微克/毫升可阻止蛙的受精卵的卵裂沟的形成,致使不能进行正常的卵裂,也抑制
Preparation of cytoplasmic extracts for the application in a cell-free system
(without Cytochalasin B). Resuspend cells in about 1 volume (same volume as cell pellet) of KPM (containing 10 µg/ml Cytochalasin B). Lyse the cells by freezing the cell-suspension in liquid nitrogen and successively thawing them in a warm water bath. Repeat
Isolation of cell nuclei for the application in the cell-free system
Posted on Monday, November 24, 2003 Description Pretreatment of epithelial cells with 20 µM Cytochalasin B for 30 min in RPMI prior to harvesting results in the reduction of cytoskeletal microfilaments and the easier release of the nuclei
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