Cell Meter活细胞Caspase 3/7与磷脂酰丝氨

【产品形式】：艾美捷为您提供的Cell Meter活细胞Caspase 3/7与磷脂酰丝氨酸PS检测试剂盒Our Cell Meter assay kits are a set of tools for monitoring cellular functions. In the process of apoptosis, one of key events is the activation of caspases. The activation of caspase 3/7 is an important for the initiation of apoptosis. It has been proven that caspase 3/7 has substrate selectivity for the peptide sequence Asp-Glu-Val-Asp (DEVD). This kit uses SR-DEVD-FMK as a fluorescent indicator to detect caspase 3/7 activities. SR-DEVD-FMK is cell permeable and nontoxic, once bound to caspases, the fluorescent reagent is retained inside the cell. The binding event prevents the caspases from further catalysis but will not stop apoptosis from proceeding. SR-DEVD-FMK is a red label reagent with Ex/Em = 550/595 nm. Annexins are a family of proteins that bind to phospholipid membranes in the presence of calcium. Annexin V is used to detect apoptotic cells that express phosphatidylserine (PS) on the cell surface. The appearance of PS on the cell surface is a universal indicator of the initial/intermediate stages of cell apoptosis. Annexin V-dye conjugates monitor cell apoptosis through measuring the translocation of PS. The Annexin V-iFluor 488 used in this kit is a green labeling reagent, with Ex/Em = 490/525 nm. The kit is designed to detect apoptosis by simultaneously monitoring Caspase 3/7 and Annexin V activities in mammalian cells. The kit also provides a Hoechst dye for labeling the nucleus of the whole population of the cells, and propidium iodide dye for staining necrosis cells. This kit is applicable for fluorescence microscope, flow cytometer, and fluorescence microplate reader. The kit provides all the essential components with an optimized assay protocol.

【背景资料】：Cell Meter检测试剂盒是一类用于检测细胞功能的系列工具，包括细胞活性、细胞毒性、细胞凋亡、细胞膜电位以及细胞周期等方面的指标。每种检测方案均能提供不同荧光颜色的检测方案。这些高效的检测方案为从多角度研究细胞功能活动提供了一种十分有效的方法。

美国AAT Bioquest Inc.(前身是ABD Bioquest，Inc.)是一家为从事生命科学研究、诊断研发及药物开发的科学家研发、生产和销售生物分析研究试剂和试剂盒的公司。公司致力于光谱学检测领域，包括显色、荧光和生物发光技术。AAT Bioquest的产品帮助全世界的科学家和生物医药研究者更好的了解生物化学，免疫学，细胞生物学和分子生物学等领域。作为AAT Bioquest Inc.的中国区域代理，艾美捷科技为中国客户提供光谱学检测技术并应用于生化、生理代谢和细胞分析领域的产品，包括显色，荧光和发光技术等全系列解决方案。

点击Cell Meter活细胞Caspase 3/7与磷脂酰丝氨酸PS检测试剂盒查看更详细产品说明，更多应用、储存、价格、货期等信息请致电垂询AAT Bioquest中国区域授权总代理艾美捷科技有限公司。