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INTERLEUKIN 6
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
西宝生物
- 英文名：
IMIDAZOLE
- 保存条件：
ROOM TEMPERATURE
- 库存：
5 kg
- 规格：
BIOCHEMICALS
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文献和实验Staining of Preparative 2-D Gels: Coomassie Blue and Imidazole-Zinc Negative Staining
-250 or negative staining with imidazole-zinc yields high-resolution stains (Fig. 1 ) that are compatible with subsequent mass spectrometric analysis ( see Notes 1 and 5 ). Fig. 1. Preparative 2-D gels stained by the imidazole-zinc
6xHis-tagged protein purification using Qiagen Ni-NTA Column
Buffers:Lysis Buffer (1 liter)50 mM NaH2PO4 6.90g NaH2PO4.H2O (MW 137.99g/mol )300 mM NaCl(up to 2M)17.54gNaCl(MW 58.44)or 60ml 5M10 mM Imidazole (up to 100mM)0.68g (MW 68.08 )10 mM BME (up to 20 mM)0.69 ml stock (14.4M )Adust pH to 8.0 using
6xHis-tagged protein purification using Qiagen Ni-NTA Column under Native Condit
Buffers:Lysis Buffer (1 liter)50 mM NaH2PO4 6.90g NaH2PO4.H2O (MW 137.99g/mol )300 mM NaCl(up to 2M)17.54gNaCl(MW 58.44)or 60ml 5M10 mM Imidazole (up to 100mM)0.68g (MW 68.08 )10 mM BME (up to 20 mM)0.69 ml stock (14.4M )Adust pH to 8.0 using
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