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蓝冰
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100 ml
ELISA Assay Diluent (5X)参数介绍>>>>
| Recommended Usage | Prior to use, dilute the ELISA Assay Diluent (5X) in PBS, pH 7.4.
|
| Applications | ELISA |
| Description | ELISA Assay Diluent is a 5x concentrated phosphate buffered saline solution containing (pre-screened) bovine serum. This buffer is specifically formulated and tested for ELISA applications, such as blocking antibody-coated ELISA plate microwells, diluting ELISA standards and samples, and diluting biotinylated detection antibodies or tertiary reagents (specifically Avidin-HRP or Streptavidin-HRP). The diluent contains a preservative compatible with peroxidase mediated enzyme activity. |
| Storage | This solution can be stored unopened at 4°C for up to the expiration date as labeled. After opening, it is recommended to use within 30 days or to be aliquoted and stored at -20°C (frost-free) or -70°C for long term storage. Warm ELISA reagents to room temperature (18-25°C) before use. |
品牌介绍:Biolegend公司自2002年成立以来,一直致力于为生物医药研究领域提供一流的,高质量的流式抗体、试剂盒及其它相关产品。公司聚集了一批来自PharMingen的专业杰出人士,建立了一支雄厚技术背景的专业队伍,并通过其自身的先天优势,以及与世界知名实验室和研究所的良好合作关系,以最合理的价格为广大客户提供卓越的免疫学和细胞生物学产品,并不断地开发新产品,以满足飞速发展的生命科学研究的需要。短短几年时间,Biolegend公司得到了飞速发展,已跻身全球最先进的抗体公司行列,并在2009年成为美国哈佛大家的独家抗体供应商! Biolegend公司的试剂主要包括细胞免疫、细胞因子、趋化因子、粘附分子等及与癌症研究、T 调节细胞研究、干细胞研究、先天免疫研究、细胞周期分析、凋亡研究及特定修饰类抗体等领域相关的试剂,用于流式、ELISA、免疫沉淀、WB、免疫荧光、免疫组化、及体内体外功能学试验。其中,Biolegend公司的流式抗体以其具有较宽范围的荧光选择及较高的性价比,为广大科研工作者所接受!同时,公司配备了一支经验丰富的技术服务队伍,为客户提供完美的配色方案及快速的售后处理服务!
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文献和实验marker, mark a second, unsensitized microtiter plate to identify the intended location of all samples to be tested, and the location of the positive and negative controls (the positive control in this assay is a purified sample of the antigen
Enzyme- Linked Immunosorbent Assay (ELISA)
Enzyme-Linked Immunosorbent Assays (ELISAs) have had many thousands of applications over the past 25 yr The technique has been used in all fields of pure and applied aspects of biology, in particular it forms the backbone of diagnostic tech
ELISA (Enzyme-Linked ImmunoadSorbent Assay)
A. You will need the following solutions PBS (Phosphate-Buffered Saline, Ca/Mg free) : Solute 1x 10x NaCl 8g 80
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