The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration
0.5mg/ml
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Applications
FC - Quality tested
Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro stimulation1,2,4 and in vivo depletion3-5. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 107506).
Application References
1. Bluestone JA, et al. 1991. Immunol. Rev. 120:5. (IP Costim) 2. Kruisbeek AM, et al. 1991. In Current Protocols in Immunology p. 3.12.1 - 3.12.14. (Costim) 3. Hiromatsu K, et al. 1992. J. Exp. Med. 175:49. (Deplete) 4. Dieli F, et al. 1997. Eur. J. Immunol. 27:206. (Costim) 5. van der Heyde HC, et al. 1995. J. Immunol. 154:3985. (Deplete) 5. Pollinger B, et al. 2011. J. Immunol. 186:2602. PubMed
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Description
T cell receptor (TCR) is a heterodimer consisting of an α and a β chain (TCR α/β) or a γ and a δ chain (TCR γ/δ). TCR γ/δ belongs to the immunoglobulin superfamily, involved in the recognition of certain bacterial and tumor antigens bound to MHC class I. The TCR γ/δ associates with CD3 and is expressed on a T cell subset found in the thymus, the intestinal epithelium, and the peripheral lymphoid tissues and peritoneum. Most γ/δ T cells are CD4-/CD8-, some are CD8+. T cells expressing the TCR γ/δ have been shown to play a role in oral tolerance, tumor-associated tolerance, and autoimmune disease. It has been reported that γ/δ T cells also play a principal role in antigen presentation. Immobilized UC7-13D5 antibody has been reported to activate TCR-γ/δ-bearing T cells in vitro, and to deplete peripheral TCR-γ/δ-bearing T cells in vivo.
Storage
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.