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FITC anti-mouse TCR γ/δ参数介绍>>>>
| Clone | UC7-13D5 |
| Isotype | Armenian Hamster IgG |
| Reactivity | Mouse |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC. |
| Concentration | 0.5mg/ml |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. |
| Applications | FC - Quality tested |
| Application Notes | Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro stimulation1,2,4 and in vivo depletion3-5. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 107506). |
| Application References | 1. Bluestone JA, et al. 1991. Immunol. Rev. 120:5. (IP Costim) |
| Description | T cell receptor (TCR) is a heterodimer consisting of an α and a β chain (TCR α/β) or a γ and a δ chain (TCR γ/δ). TCR γ/δ belongs to the immunoglobulin superfamily, involved in the recognition of certain bacterial and tumor antigens bound to MHC class I. The TCR γ/δ associates with CD3 and is expressed on a T cell subset found in the thymus, the intestinal epithelium, and the peripheral lymphoid tissues and peritoneum. Most γ/δ T cells are CD4-/CD8-, some are CD8+. T cells expressing the TCR γ/δ have been shown to play a role in oral tolerance, tumor-associated tolerance, and autoimmune disease. It has been reported that γ/δ T cells also play a principal role in antigen presentation. Immobilized UC7-13D5 antibody has been reported to activate TCR-γ/δ-bearing T cells in vitro, and to deplete peripheral TCR-γ/δ-bearing T cells in vivo. |
| Storage | The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. |
品牌介绍:Biolegend公司自2002年成立以来,一直致力于为生物医药研究领域提供一流的,高质量的流式抗体、试剂盒及其它相关产品。公司聚集了一批来自PharMingen的专业杰出人士,建立了一支雄厚技术背景的专业队伍,并通过其自身的先天优势,以及与世界知名实验室和研究所的良好合作关系,以最合理的价格为广大客户提供卓越的免疫学和细胞生物学产品,并不断地开发新产品,以满足飞速发展的生命科学研究的需要。短短几年时间,Biolegend公司得到了飞速发展,已跻身全球最先进的抗体公司行列,并在2009年成为美国哈佛大家的独家抗体供应商! Biolegend公司的试剂主要包括细胞免疫、细胞因子、趋化因子、粘附分子等及与癌症研究、T 调节细胞研究、干细胞研究、先天免疫研究、细胞周期分析、凋亡研究及特定修饰类抗体等领域相关的试剂,用于流式、ELISA、免疫沉淀、WB、免疫荧光、免疫组化、及体内体外功能学试验。其中,Biolegend公司的流式抗体以其具有较宽范围的荧光选择及较高的性价比,为广大科研工作者所接受!同时,公司配备了一支经验丰富的技术服务队伍,为客户提供完美的配色方案及快速的售后处理服务!
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文献和实验癌 (NCT00562666)、肾癌[19,20]、乳腺癌[21]、前列腺癌[10]、神经母细胞瘤[21]和肺癌[23,24]等。这些临床研究证实了γδ T细胞的过继性转移是安全可行的[25]。 而本文开头提到的Adicet Bio公司正在开发的产品通过anti-CD20 CAR和γδ T细胞内源性细胞毒性受体靶向恶性B细胞,在临床前模型中表现出高效的抗肿瘤活性,有效地控制了肿瘤的生长。 图3. Adicet基于γδ T细胞的细胞疗法流程(图片来源于Adicet官网) 目前γδ T细胞疗法面临的最大阻碍包括
1.1 材料和仪器RPMI-1640(Gibco公司产品),人AB血清来自徐州市血站,胎牛血清和淋巴细胞分离液购自中国科学院血液病研究所,异戊烯焦磷酸(isopentenylpgrophosphate.IPP)购自sigma公司,IL-2(夏门),抗人TCR-γδ-FITC,抗人CD3Ab-PE,抗人CD44Ab-FITC,均购自杭州联科生物(Immutech,French),K562细胞和SGC-7901胃腺癌细胞由中国科学院上海细胞所提供,流式细胞分析仪(FACSC alibur.Becton
【进展|热点】Immunity: γδ T细胞抑制调节性T细胞
γδ T细胞抑制调节性T细胞 文章出处:γδ T Cells Enhance Autoimmunity by Restraining Regulatory T Cell Responses via an Interleukin-23-Dependent Mechanism. Immunity. 2010. 33:351-363 主要内容: T细胞根据其TCR链的不同可以分为αβ T细胞和γδ T细胞两类,前者是经典的T细胞,我们常规提到的T细胞指的就是αβ T细胞。γδ
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