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- 保存条件:
蓝冰
- 规格:
25 ug
APC anti-DYKDDDDK参数介绍>>>>
| Clone | L5 |
| Isotype | Rat IgG2a, κ |
| Reactivity | DYKDDDDK tag epitope |
| Immunogen | DYKDDDDK-tagged mouse Langerin |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody. |
| Concentration | 0.2mg/ml |
| Recommended Usage | Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. |
| Applications | FC, ICFC - Quality tested |
| Application Notes | The L5 clone has been demonstrated to have 2-8 fold better sensitivity in WB than another commonly used antibody clone, M2. |
| Application References | 1. Park SH, et al. 2008. J Immunol Methods. 331:27. |
| Description | The DYKDDDDK tag, commonly referred to as Sigma®'s FLAG® Tag, is often used as a protein modification in order to simplify the labeling and detection of proteins. This unique amino acid sequence allows for specific antibody detection in western blotting, immunoprecipitation, and immunostaining techniques. Due to the short sequence, this modification is not likely to affect the structure or function of the modified proteins. |
| Storage | The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. |
品牌介绍:Biolegend公司自2002年成立以来,一直致力于为生物医药研究领域提供一流的,高质量的流式抗体、试剂盒及其它相关产品。公司聚集了一批来自PharMingen的专业杰出人士,建立了一支雄厚技术背景的专业队伍,并通过其自身的先天优势,以及与世界知名实验室和研究所的良好合作关系,以最合理的价格为广大客户提供卓越的免疫学和细胞生物学产品,并不断地开发新产品,以满足飞速发展的生命科学研究的需要。短短几年时间,Biolegend公司得到了飞速发展,已跻身全球最先进的抗体公司行列,并在2009年成为美国哈佛大家的独家抗体供应商! Biolegend公司的试剂主要包括细胞免疫、细胞因子、趋化因子、粘附分子等及与癌症研究、T 调节细胞研究、干细胞研究、先天免疫研究、细胞周期分析、凋亡研究及特定修饰类抗体等领域相关的试剂,用于流式、ELISA、免疫沉淀、WB、免疫荧光、免疫组化、及体内体外功能学试验。其中,Biolegend公司的流式抗体以其具有较宽范围的荧光选择及较高的性价比,为广大科研工作者所接受!同时,公司配备了一支经验丰富的技术服务队伍,为客户提供完美的配色方案及快速的售后处理服务!
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文献和实验Anti-DYKDDDDK tag (L5) Affinity Gel
or 0.22µm filter to prepare for L5 purification. Part II. Resin Preparation 1. Thoroughly suspend the resin by gentle inversion until the bottle of Anti- DYKDDDDK-tag (L5) affinity gel is a uniform suspension of gel beads. Transfer
酶活性的DNA聚合酶,原来通常使用的是Taq DNA聚合酶,缺少校正功能。在这儿我们展示将长PCR技术应用到5'RACE系统中,来分离5.5kb长的人结节性脑硬化II(tuberous sclerosis II,TSC-2)mRNA和8.9kb长的腺瘤性结肠息肉(adenomatous polyposis coli,APC)mRNA的5'末端,扩增的5'末端片段要比单独用Taq DNA聚合酶获得的片段长。方法使用5'RACE系统版本2 (Cat. No. 18374-058)来扩增TSC-2和APC
APC 由阿斯匹林( Aspirin )、非那西汀( Phenacetin )与咖啡因( Caffeine )组成。它有三个主要的生理功能:止痛、退热降温和消炎(如活动性风湿症、类风湿性关节炎)。阿斯匹林的化学名为乙酰水杨酸,由水杨酸(即邻羟基苯甲酸)与醋酐反应而制得: 其实,水杨酸本身已具有上述生理功能,但酚基会刺激人的嘴和咽喉。在水杨酸分子中— OH 被转变为 基,便消除了水杨酸的副作用。服用后进入消化系统,它又转变成水杨
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