FITC anti-human CD45RA

FITC anti-human CD45RA

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  • ¥100 - 9999
  • Biolegend
  • 美国
  • 2025年07月12日
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    • 详细信息
    • 技术资料
    • 保存条件

      蓝冰

    • 规格

      100 tests

    上海科敏生物经销国内外知名品牌试剂抗体,折扣低,货期快,欢迎新老客户咨询订购!
    FITC anti-human CD45RA参数介绍>>>>
    Clone HI100
    Isotype Mouse IgG2b, κ
    Reactivity Human, Cross-Reactivity: Chimpanzee
    Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
    Preparation The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
    Recommended Usage

    Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.

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    Applications

    FC - Quality tested

    Application Notes

    Additional reported applications (for relevant formats of this clone) include: inhibition of CD45 functions2, and immunohistochemical staining of frozen tissue sections3 and formalin-fixed paraffin-embedded tissue sections4.

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    Application References

    1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
    2. Yamada T, et al. 2002. J. Biol. Chem. 277:28830. (WB, Block)
    3. Weninger W, et al. 2003 J. Immunol. 170:4638. (IHC)
    4. Imanguli MM, et al. 2009. Blood. 113:3620 (IHC)
    5. Roque S, et al. 2007. J. Immunol. 178:8028. (FC) PubMed
    6. Smeltz RB. 2007. J. Immunol. 178:4786. (FC) PubMed
    7. Palendira U, et al. 2008. Blood (FC) PubMed
    8. Kuttruff S, et al. 2009. Blood 113:358. (FC) PubMed
    10. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
    11. Alanio C, et al. 2010. Blood 115:3718. (FC) PubMed
    12. Iannello A, et al. 2010. J. Immunol. 184:114. (FC) PubMed
    13. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
    14. Guereau-de-Arellan M, et al. 2011. Brain. PubMed.

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    Description

    CD45RA is a 205-220 kD single chain type I glycoprotein. It is an exon 4 splice variant of the tyrosine phosphatase CD45. The CD45RA isoform is expressed on resting/naïve T cells, medullary thymocytes, B cells and monocytes. CD45RA enhances both T cell receptor and B cell receptor signaling. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4. CD45 has also been reported to bind galectin-1. CD45 isoform expression can change in response to cytokines.

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    Storage The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

    品牌介绍:Biolegend公司自2002年成立以来,一直致力于为生物医药研究领域提供一流的,高质量的流式抗体、试剂盒及其它相关产品。公司聚集了一批来自PharMingen的专业杰出人士,建立了一支雄厚技术背景的专业队伍,并通过其自身的先天优势,以及与世界知名实验室和研究所的良好合作关系,以最合理的价格为广大客户提供卓越的免疫学和细胞生物学产品,并不断地开发新产品,以满足飞速发展的生命科学研究的需要。短短几年时间,Biolegend公司得到了飞速发展,已跻身全球最先进的抗体公司行列,并在2009年成为美国哈佛大家的独家抗体供应商! Biolegend公司的试剂主要包括细胞免疫、细胞因子、趋化因子、粘附分子等及与癌症研究、T 调节细胞研究、干细胞研究、先天免疫研究、细胞周期分析、凋亡研究及特定修饰类抗体等领域相关的试剂,用于流式、ELISA、免疫沉淀、WB、免疫荧光、免疫组化、及体内体外功能学试验。其中,Biolegend公司的流式抗体以其具有较宽范围的荧光选择及较高的性价比,为广大科研工作者所接受!同时,公司配备了一支经验丰富的技术服务队伍,为客户提供完美的配色方案及快速的售后处理服务!

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