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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
Luciferase Cell Lysis Buffer
- 供应商:
南京赛泓瑞
- 规格:
25.0 ml
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文献和实验ChIP Protocol-Mechanical Breakage & FA Lysis Buffer
ChIP Protocol-Mechanical Breakage & FA Lysis Buffer Day -2: o Start a 5ml overnight culture from a single colony. Day -1: o Start a larger overnight culture using part of the 5ml culture (subculture). Day
Jacobs:Protocol Total Protein Isolation Using RIPA Lysis Buffer
cells twice with PBS pouring excess off into waste beaker Carefully soak up any extra PBS with a Kimwipe Add 150ul of RIPA lysis buffer to the culture dish Use cell scraper to scrape cells from the bottom of the dish Pass cell
Cell Lysis/Western/IPSteven Finkbeiner,Departments of Neurology and Physiology,UCSFcompiled by 6/95 by AJS from AB)Fresh 1X LysisFresh 1X LysisFinal5ml10ml2X Lysis 2.5 ml5mlNaCl0.25M0.25ml of 5M0.5 ml of 5MNa3VO40.1mM25l (μl) of 200 mM5l (μl) of 200
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