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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 英文名：
Anti asialo GM1 (Rabbit)
- 保存条件：
冷藏
- 规格：
1ML
Anti Asialo GM1 antiserum has been shown to eliminate natural killer (NK) activity in cells of various strains of mice and rats.
Each package comes with in vivo and in vitro titration data for the lot.
PREPARATION:
Repeated immunization was performed with purified Asialo GM1 from bovine brain tissue, in conjunction with methylated bovine serum albumin and complete Freund's adjuvant. Gammaglobulin fraction of serum was obtained by 50% ammonium sulfate precipitation methods followed by dialysis with phosphate buffered saline (pH 7.2).
SPECIFICITY:
Reacts with mouse and rat NK cells; mouse monocytes (liver cells which contain no NK cells; bone marrow; fetal liver cells; spleen cells of nude mice-macrophages); fetal thymocytes (12 days old; ratio of existence decreased gradually until there were none in newborn mice).
IMMUNOGLOBULIN FRACTIONS: IgG, IgA, and IgM.
RECONSTITUTION:
Distilled water is recommended (1 mL). Since the material is lyophilized with salts, use of other solvents such as PBS or MEM may increase the salt concentration.
ANTIBODY TITER:
Approximately 1 : 1,000 by immunoflocculation test
To get further information, please visit
http://www.wako-chem.co.jp/english/labchem/pdf/98-1000_AAGM1.pdf
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- 作者
- 内容
- 询问日期
文献和实验功能。 8、RORγt- NKR-LTi细胞是实验性固有性肠炎的强力启动者。 方法借鉴： 1、实验性固有性肠炎模型制作方法：Rag2缺陷小鼠注射抗CD40抗体。 2、genetic lineage tracing法鉴定细胞的分化来源。 3、体内去除NK细胞的方法：注射anti-asialo-GM1。
Human Gangliosides and Bacterial Lipo-oligosaccharides in the Development of Autoimmune Neuropathies
Guillain–Barr� syndrome (GBS), the most frequent cause of acute flaccid paralysis, can develop after infection by Campylobacter jejuni . The condition is often associated with serum anti-GM1 or anti-GD1a IgG antibodies. Gangliosides
Isolation of Membrane Rafts and Signaling Complexes
, and Tritoninsoluble floating fraction. They are enriched in cholesterol, often sphingomyelin and various gangliosides (GM1, GM2, and GM3). The ability of the B-subunit of cholera toxin to bind GM1 has been exploited to visualize membrane rafts by confocal microscopy
