DIG-High Prime DNA Labeling and Detection Starter Kit I
For 12 random-primed DNA labeling reactions with DIG-11-dUTP, alkali-labile, and 24 color detection reactions with NBT/BCIP.
For life science research only. Not for use in diagnostic procedures.
Convenient kit for random-primed labeling of DNA templates with DIG-11-dUTP, alkali-labile, and color detection of the DIG-labeled hybrids. In this method, the complementary DNA strand of denatured DNA is synthesized by Klenow polymerase using the 3'-OH termini of the random oligonucleotides as primers.This kit was assembled with convenience in mind, offering a ready-made blocking solution, combined stock solution of NBT/BCIP, and DIG Easy Hyb granules. The DIG-High Prime mixture includes stabilized Klenow Enzyme, nucleotides, primers, and reaction buffer, all in one convenient reagent.
Contents
DIG-High Prime, 5x concentrated
DIG-labeled Control DNA (5 µg/ml), pBR328 (linearized with BamH I)