- ¥3798
- Roche
- 11745832910
- 2025年07月15日
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50T
DIG-High Prime DNA Labeling and Detection Starter Kit I
For 12 random-primed DNA labeling reactions with DIG-11-dUTP, alkali-labile, and 24 color detection reactions with NBT/BCIP.
For life science research only. Not for use in diagnostic procedures.
Convenient kit for random-primed labeling of DNA templates with DIG-11-dUTP, alkali-labile, and color detection of the DIG-labeled hybrids. In this method, the complementary DNA strand of denatured DNA is synthesized by Klenow polymerase using the 3'-OH termini of the random oligonucleotides as primers.This kit was assembled with convenience in mind, offering a ready-made blocking solution, combined stock solution of NBT/BCIP, and DIG Easy Hyb granules. The DIG-High Prime mixture includes stabilized Klenow Enzyme, nucleotides, primers, and reaction buffer, all in one convenient reagent.
Contents
- DIG-High Prime, 5x concentrated
- DIG-labeled Control DNA (5 µg/ml), pBR328 (linearized with BamH I)
- DNA Dilution Buffer
- Anti-digoxigenin-AP Conjugate
- NBT/BCIP Stock Solution, concentrated
- Blocking Solution, 10x concentrated
- DIG Easy Hyb Granules
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文献和实验标记DNA探针 如果您有足够量的高纯度模板，建议您采用随机引物标记方法的DIG High Prime DNA Labeling and Detection Starter Kit I 和II。这两个试剂盒包含了标记、封闭、杂交和检测所需要的所有试剂，是真正意义上的一站式服务的试剂盒。二者之间的差别在于检测方法的不同：Starter Kit
, or Fluorochromes Procedures for Labeling DNA, RNA, and Oligonucleotides with DIG, Biotin, or Fluorochromes 41 Random primed labeling of ds DNA with DIG-, Biotin- or Fluorescein-High Prime reaction mix 41 PCR labeling of ds DNA with the PCR DIG Probe Synthesis Kit
【建议】Southern和Northern中的奇怪问题？请大家讨论。
的DIG Northern Starter Kit，没有用他的显色底物，而是用的DIG High Prime DNA Labeling and Detection Starter Kit I中的NBT/BCIP底物，因为我没有显影液和定影液，所以没有用CDP-Star。还有为什么在边缘的条带跑的快，是不是MOPS电泳缓冲液的原因，我在整个电泳过程中没有换过缓冲液，电泳大约2个小时左右，没40分钟停止电泳，拿出胶后搅一搅缓冲液。 第二张图是Southern杂交，用的是DIG High Prime
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