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TRYPSIN
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
ROOM TEMPERATURE
- 供应商:
西宝生物
- 库存:
10x1.0 L
- 英文名:
SD MEDIUM-ADE-HIS-LEU-TRP (PACK)(POUCHES)
- 规格:
MOLECULAR BIOLOGY
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文献和实验, 琼脂 20g ,缺相应氨基酸的培养基 所要求的质量(-Ade/-His/-Leu/-Trp DO Supplement:0.6g;-Leu/-Trp DO Supplement:0.64g;DH2O 850ml, -Leu DO Supplement:0.69g)超净台内加入所要补充的氨基酸 (Ade,His,Leu,Trip)10×Dropout solution 100ml ,葡萄糖 20g或灭菌后加入葡萄糖(40% 50ml;过滤除菌/100~110℃,30min),使终浓度为2%.成分
Membrane‐Based Yeast Two‐Hybrid System to Detect Protein Interactions
) 10‐cm diameter plates containing SD solid medium lacking tryptophan, leucine, histidine, or adenine (SD−Trp−Leu, SD−Trp−Leu−His, and SD−Trp−Leu−His−Ade; see recipe )
for 5 sec at room temperature at 14 K rpm 21. Remove the supernatant. 22. Resuspend cells in 0.5 ml of 1X TE b : 23. Proceed to plating (on either SD �Trp/�Leu) or SD �Trp/�Leu/�His/�Ade with or without X- á -gal as required.
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