Description Pyruvate is a key intermediate in cellular metabolic pathways. Pyruvate can be converted to carbohydrates via gluconeogenesis, to fatty acids or energy through acetyl-CoA, to the amino acid alanine and to ethanol. Abnormal levels of pyruvate have been linked to liver diseases and metabolic disorders. Simple, direct and automation-ready procedures for measuring pyruvate concentrations find wide applications in research and drug discovery. our pyruvate assay uses a single Working Reagent that combines pyruvate oxidase and hydrogen peroxide determination in one step. The color intensity of the reaction product at 570nm or fluorescence intensity at lem/ex = 585/530nm is directly proportional to pyruvate concentration in the sample.
Key features Sensitive and accurate. Use as little as 10 mL samples. Linear detection range in 96-well plate: 2 to 500 mM (17 mg/dL to 4.4 mg/dL) pyruvate for colorimetric assays and 0.2 to 50 mM for fluorimetric assays.Simple and convenient. The procedure involves addition of a single working reagent and incubation for 30 min at room temperature, compatible for HTS assays.Improved reagent stability. The optimized formulation has greatly enhanced the reagent and signal stability.
Applications: Direct Assays: pyruvate in biological samples.Drug Discovery/Pharmacology: effects of drugs on pyruvate metabolism.
Storage conditions. The kit is shipped on dry ice. Store all reagents at -20°C. Shelf life of three months after receipt.
其他 : 编号:EPYR-048,EPYR-100
测试:48T,100T