产品特点 敏感、准确: 96-孔板检测范围为0.05~2 mM乳酸,对于含有酚红的细胞培养物样品,检测范围为0.1 mM~1 mM 乳酸。方便: 仅需添加单一工作试剂并在开始和20分钟时读取光密度。室温下检测,无须37℃加热器。高通量: 通过96-孔板高通量的自动化检测,每天可以检测上千份样品。
贮藏条件:所有试剂均于-20°C保存。
Description Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. Simple, direct and automation-ready procedures for measuring lactate concentration are very desirable. our EnzyChromTM lactate assay kit is based on lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a formazan (MTT) reagent. The intensity of the product color, measured at 565 nm, is proportionate to the lactate concentration in the sample.
Applications Direct Assays: lactate in serum, plasma, and cell media samples.
Key features Sensitive and accurate. Detection limit of 0.05 mM and linearity up to 2 mM L-Lactate in 96-well plate assay. For cell culture samples containing phenol red: detection limit of 0.1 mM and linearity up to 1 mM L-Lactate in 96-well plate assay.Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and at 20 min. Room temperature assay. No 37°C heater is needed.High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
Storage conditions. Store all reagents at -20°C. Shelf life of at least 6 months (see expiry dates on labels).