Description CATALASE (EC 1.11.1.6), is an ubiquitous antioxidant enzyme that catalyzes the decomposition of hydrogen peroxide (H2O2) to water and oxygen.By preventing excessive H2O2 build up, catalase allows important cellular processes which produce H2O2 as a byproduct to occur safely. Deficiency in catalase activity has been associated with grey hair and peroxisomal disorder acatalasia. Simple, direct and high-throughput assays for catalase activity find wide applications. our improved assay directly measures catalase degradation of H2O2 using a redox dye. The change in color intensity at 570nm or fluorescence intensity (lem/ex = 585/530nm) is directly proportional to the catalase activity in the sample.
Key features Sensitive and accurate. Use 10 mL sample. Linear detection range 0.2 to 5 U/L catalase activity.Simple and Convenient. The procedure involves adding a Substrate to sample, incubation for 30 min, followed by a Detection Reagent and reading the optical density or fluorescence intensity.
Applications: Direct Assays: catalase activity in biological samples e.g. serum, plasma, urine, saliva, cell culture etc.Drug Discovery/Pharmacology: effects of drugs on catalase activity.
Storage conditions. The kit is shipped on ice. Store all components at -20°C. Shelf life of three months after receipt.