荧光素酶报告基因测试盒 | SuperLight™ Luci

产品名称：
荧光素酶报告基因测试盒 | SuperLight™ Luciferase Reporter Gene Assay Kit

分类名称 :
高效药物筛选

产品介绍 :
测定方法 快速（2分钟内）生物发光法测定荧光素酶报告基因在细胞中的表达。

Description The SuperLight^TM Luciferase Reporter Gene Assay is based on the quantitation of luciferase expression in mammalian, yeast or E. coil cells, using luciferin and ATP as substrates. The reaction results in light production which can be conveniently measured on a luminometer Reaction scheme:This bioluminescent reporter gene assay is extremely sensitive and is especially suitable for quantifying luciferase expression in recombinant cells. This ultra-sensitive, homogeneous cell-based assay only requires adding a single reagent to the cells and measuring the light intensity after a short incubation step (2 minutes). Assays can be performed in tubes, cuvettes or multi-well plates. All kit components are compatible with culture media and with all liquid handling systems. With an extended luminescence emission kinetics (half-life 40 min), the SuperLight^TM luciferase assays are especially suitable for high-throughput screening in 96-well, 384-well and 1536-well plates. In addition, the reagent provided in the kits has been formulated for maximum sensitivity, reproducibility and long shelf-life. Applications for this kiinclude gene t regulation studies and high-throughput screening of gene modulators.

Key features High sensitivity and wide detection range: detection of as little of 2 fg luciferase and as few as 4 cells. Plus, the emitted light is linear over seven orders of magnitude.Compatible with routine laboratory and HTS formats: assays can be performed in tubes or microplates, on LJL Analyst, Berthold Luminometer, Top-Count, MicroBeta counters, chemiluminescent image plate readers (CLIPR/LeadSeeker). Assay reagents compatible with all liquid handling systems.Fast and convenient: homogeneous “mix-and-measure” assay allows detection of luciferase levels within 10 minutes. The optimally combined reagent system allows a single addition step, and simultaneous cell lysis and detection.Robust and amenable to HTS: Z’ factors of 0.6 to 0.8 are observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems.

Applications Gene Regulation: gene expression level, characterization of promoter activity, modulation of gene expression by receptors, transcription factors and small molecules.Drug Discovery: high-throughput screen for gene modulators.

Storage conditions. Store the Reagent in the provided amber tube at -20°C and the Assay Buffer at 2-8°C. Shelf life: 12 month.

其他 :
编号：SLLU-048，SLLU-200
测试：48，200T

公司简介：
博世生物提供优质、快速(如2分钟)、方便的生化检测试剂盒，广泛适用于人和动物等生物样品、食品和环境监测样品中相关指标的检测。博世专注于无害、无放 射性的检测技术(如吸收光、荧光、生物化学发光法等)，安全可靠，适合各类仪器(分光光度仪、微孔读数仪等）。产品具有快速、简单、高通量（可用于HTS 药物筛选）、灵敏(特别适合小鼠样品) 、准确性高和低干扰等主要特征。检测步骤简便，用户不需花额外的时间来作调适测试。
博世生物产品主要包括：1、血液尿液化学；2、酶活性测定；3、能量代谢；4、氧化应激；5、高通量HTS试剂；6、阴阳离子检测。