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文献和实验【求助】有什么办法可以阻滞LPS刺激的TNF-alpha分泌?
sonny_zan 我实验的关键步骤,现在就是不知道有什么办法可以阻滞LPS刺激后PBMC分泌TNF-alpha,考虑过用p38 MAPK抑制剂SB203580,后来查到一篇文献说SB203580对LPS刺激后p38 MAPK的活性影响甚微或无。 请各位大侠给点意见啦!我快急死了~~~ xiongran 拿抗体把PBMC表面的TLR4封闭掉试试看! pureclear 可以考虑
There is an increasing interest in the preparation of rat � rat hybridomas, because they have been found to be more stable in culture than mouse hybridomas and they secrete consistently high levels (10 �g/mL and above) of monoclonal antibody
The first transgenic experiment was carried out more than 15 yr ago, and transgenesis is now a routine technique for the study of gene and cell function, and the development of animal models of disease. The mouse remains the species of choice
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