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文献和实验Assays for GH, IGF-I, and IGF Binding Protein-3
binding protein-3). Enzyme-linked immunosorbent assays (ELISAs) are described as they combine the specificity of antibody–antigen interactions with the sensitivity of simple enzyme assays. Many commercial immunoassays are available for the measurement
Purification of the human TATA-Binding Protein, TBP
The TATA-binding protein TBP plays a central role in eukaryotic transcription initiation (1 , 2 ). TBP binds to the promoter consensus sequence TATAAA, located approx 30 nucleotides upstream of the transcriptional start site. Once bound, TBP
Secretion of Recombinant Human Insulin-Like Growth Factor I (IGF-I)
The development of efficient recombinant protein production processes can be a critical factor in whether or not a pharmaceutical therapeutic protein can enter human clinical trials and ultimately the marketplace. This is especially true
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