Alexa Fluor® 350 NHS Ester (Su

Alexa Fluor® 350 NHS Ester (Succinimidyl Ester)

Alexa Fluor® 350 NHS Ester (Succinimidyl Ester)

(Molecular Probes®)

描述

Alexa Fluor® 350 is a blue-fluorescent dye. Used for stable signal generation in imaging and flow cytometry, Alexa Fluor® 350 dye is water soluble and pH-insensitive from pH 4 to pH 10. In addition to reactive dye formulations, we offer Alexa Fluor® 350 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection
The NHS ester (or succinimidyl ester) of Alexa Fluor® 350 is the most popular tool for conjugating this dye to a protein or antibody. NHS esters can be used to label to the primary amines (R-NH₂) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting Alexa Fluor® conjugate will exhibit brighter fluorescence and greater photostability than the conjugates of other spectrally similar fluorophores.

Detailed information about this AlexaFluor® NHS ester:

Fluorophore label: Alexa Fluor® 350 dye
Reactive group: NHS ester
Reactivity: Primary amines on proteins and ligands, amine-modified oligonucleotides
Ex/Em of the conjugate: 346/445 nm
Extinction coefficient: 19,000 cm-1M-1
Spectrally similar dyes: Marina Blue
Molecular weight: 410.4

Typical Conjugation Reaction
You can conjugate amine-reactive reagents with virtually any protein or peptide (the provided protocol is optimized for IgG antibodies). You can scale the reaction for any amount of protein, but the concentration of the protein should be at least 2 mg/mL for optimal results. We recommend trying three different degrees of labeling, using three different molar ratios of the reactive reagent to protein.

The Alexa Fluor® NHS ester is typically dissolved in high-quality anhydrous dimethylformamide (DMF) or dimethylsulfoxide (DMSO) (D12345), and the reaction is carried out in 0.1–0.2 M sodium bicarbonate buffer, pH 8.3, at room temperature for 1 hour. Because the pKa of the terminal amine is lower than that of the lysine epsilon-amino group, you may achieve more selective labeling of the amine terminus using a buffer closer to neutral pH.
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常用规格

Label or Dye:	Alexa Fluor® 350
Chemical Reactivity:	Amine
Product Size:	5 mg
Shipping Condition:	Room Temperature
Regulatory Statement:	For Research Use Only. Not for use in diagnostic procedures.