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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Brefeldin A Solution (1000X)
- 规格：
1ml
of cells in culture with Brefeldin A leads to blockade of protein transport
to the Golgi complex (GC) and accumulation of proteins in the endoplasmic
reticulum (ER). Addition of Brefeldin A during the last hours of in vitro
activation of cells results in enhanced detection of intracellular cytokines.
Brefeldin A is effective for enhanced detection of a majority of mouse and
human intracellular cytokines; however, it is advised that the investigators
evaluate the use and efficacy of this reagent as well as other protein transport
inhibitors such as Monensin in their specific assay system.
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文献和实验PREPARATION OF 2% FORMALDEHYDE STOCK SOLUTION (2 METHODS)
of 1 X PBS. Heat to 70 ° C (do not exceed this temperature) in a fume hood until the paraformaldehyde goes into solution (note that this happens quickly as soon as the suspension reaches 70 ° C). Allow the solution to cool to room temperature. Adjust
PREPARATION OF 2% FORMALDEHYDE STOCK SOLUTION (2 METHODS)
of 1 X PBS. Heat to 70 ° C (do not exceed this temperature) in a fume hood until the paraformaldehyde goes into solution (note that this happens quickly as soon as the suspension reaches 70 ° C). Allow the solution to cool to room temperature. Adjust
扎培克氏溶液 Czapek ’ s solution 指用于培养真菌特别是霉菌所用的基础合成培养基（ J ． Czapek ， 1901 ）。其组成为蔗糖 30 克、 NaN0 3 2 克、 KH 2 PO 4 1 克、 MgSO 4 · 7 HO 2 0.5 克、 KCl 0.5 克、 FeSO 4 0.01 克。水 1000 毫升。（若配制固体培养基时，则加入琼脂 20 克）
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