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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Monensin Solution (1000X)
- 规格：
1ml
of cells in culture with Monensin leads to blockade of protein transport
to the Golgi complex (GC) and accumulation of proteins in the endoplasmic
reticulum (ER). Addition of Monensin during the last hours of in vitro
activation of cells results in enhanced detection of intracellular cytokines.
Monensin is effective for enhanced detection of a majority of mouse and human
intracellular cytokines; however, it is advised that the investigators evaluate
the use and efficacy of this reagent as well as other protein transport inhibitors
such as Brefeldin A in their specific assay system.
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文献和实验PREPARATION OF 2% FORMALDEHYDE STOCK SOLUTION (2 METHODS)
of 1 X PBS. Heat to 70 ° C (do not exceed this temperature) in a fume hood until the paraformaldehyde goes into solution (note that this happens quickly as soon as the suspension reaches 70 ° C). Allow the solution to cool to room temperature. Adjust
PREPARATION OF 2% FORMALDEHYDE STOCK SOLUTION (2 METHODS)
of 1 X PBS. Heat to 70 ° C (do not exceed this temperature) in a fume hood until the paraformaldehyde goes into solution (note that this happens quickly as soon as the suspension reaches 70 ° C). Allow the solution to cool to room temperature. Adjust
扎培克氏溶液 Czapek ’ s solution 指用于培养真菌特别是霉菌所用的基础合成培养基（ J ． Czapek ， 1901 ）。其组成为蔗糖 30 克、 NaN0 3 2 克、 KH 2 PO 4 1 克、 MgSO 4 · 7 HO 2 0.5 克、 KCl 0.5 克、 FeSO 4 0.01 克。水 1000 毫升。（若配制固体培养基时，则加入琼脂 20 克）
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