Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
规格:
96T
白介素29(IL29)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Interleukin 29 (IL29)
产品编号 SEC029Hu 适用生物 Homo sapiens (Human,人)
品牌商 Cloud-Clone Corp. (美国)
检测范围 7.81-500pg/mL 灵敏度 2.8pg/mL
样本类型 Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
实验方法 双抗夹心法
Recovery
Matrices listed below were spiked with certain level of recombinant Interleukin 29 (IL29) and the recovery rates were calculated by comparing the measured value to the expected amount of Interleukin 29 (IL29) in samples.
Matrix
Recovery range (%)
Average(%)
serum(n=5)
99-105
102
EDTA plasma(n=5)
97-105
101
heparin plasma(n=5)
94-104
101
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 29 (IL29) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 29 (IL29) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Interleukin 29 (IL29) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
89-97%
83-92%
80-96%
87-101%
EDTA plasma(n=5)
92-101%
97-105%
96-104%
92-101%
heparin plasma(n=5)
87-94%
79-101%
95-105%
80-101%
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.