白介素22检测试剂盒

This assay has high sensitivity and excellent specificity for detection of Interleukin 22 (IL22).
No significant cross-reactivity or interference between Interleukin 22 (IL22) and analogues was observed.

Matrices listed below were spiked with certain level of recombinant Interleukin 22 (IL22) and the recovery rates were calculated by comparing the measured value to the expected amount of Interleukin 22 (IL22) in samples.

Matrix	Recovery range (%)	Average(%)
serum(n=5)	91-98	95
EDTA plasma(n=5)	90-97	94
heparin plasma(n=5)	96-103	99

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Interleukin 22 (IL22) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8	1:16
serum(n=5)	97-104%	78-104%	81-104%	80-93%
EDTA plasma(n=5)	84-99%	78-97%	89-103%	94-105%
heparin plasma(n=5)	84-101%	97-105%	88-103%	87-95%

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents	Quantity	Reagents	Quantity
Pre-coated, ready to use 96-well strip plate	1	Plate sealer for 96 wells	4
Standard	2	Standard Diluent	1×20mL
Detection Reagent A	1×120µL	Assay Diluent A	1×12mL
Detection Reagent B	1×120µL	Assay Diluent B	1×12mL
TMB Substrate	1×9mL	Stop Solution	1×6mL
Wash Buffer (30 × concentrate)	1×20mL	Instruction manual	1

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37^oC;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37^oC;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37^oC;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37^oC;
8. Add 50µL Stop Solution. Read at 450nm immediately.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 22 (IL22). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 22 (IL22). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 22 (IL22), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 22 (IL22) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

产品编号	中文名称		英文名称	适用生物
SEC032Hu	白介素22(IL22)检测试剂盒(酶联免疫吸附试验法)		ELISA Kit for Interleukin 22 (IL22)	Homo sapiens (Human)
SEC032Mu	白介素22(IL22)检测试剂盒(酶联免疫吸附试验法)		ELISA Kit for Interleukin 22 (IL22)	Mus musculus (Mouse)
SEE767Ra	白介素22受体α2(IL22Rα2)检测试剂盒(酶联免疫吸附试验法)		ELISA Kit for Interleukin 22 Receptor Alpha 2 (IL22Ra2)	Rattus norvegicus (Rat)
SEE767Hu	白介素22受体α2(IL22Rα2)检测试剂盒(酶联免疫吸附试验法)		ELISA Kit for Interleukin 22 Receptor Alpha 2 (IL22Ra2)	Homo sapiens (Human)
SEE767Mu	白介素22受体α2(IL22Rα2)检测试剂盒(酶联免疫吸附试验法)		ELISA Kit for Interleukin 22 Receptor Alpha 2 (IL22Ra2)	Mus musculus (Mouse)
SCC032Hu	白介素22(IL22)检测试剂盒(化学发光免疫分析法)		CLIA Kit for Interleukin 22 (IL22)	Homo sapiens (Human)
SCC032Mu	白介素22(IL22)检测试剂盒(化学发光免疫分析法)		CLIA Kit for Interleukin 22 (IL22)	Mus musculus (Mouse)
SEC032Bo	白介素22(IL22)检测试剂盒(酶联免疫吸附试验法)		ELISA Kit for Interleukin 22 (IL22)	Bos taurus; Bovine (Cattle)