Life Technologies 大家庭中的细胞培养产品。 Life Technologies公司已准备就绪,以满足您的细胞培养需要一个全面的选择,包括培养基,营养添加剂,盐溶液,缓冲液,化学物质,解离试剂和转染的 试剂。您可以依靠严格的质量控制和技术支持,以保证您的满意。仅供研究使用。 不用于任何动物或人类治疗或诊断使用。
详细说明
常用规格
Chelators:
EDTA
Phenol Red Indicator:
Phenol Red
Classification:
Animal Origin
Form:
Liquid
pH Range:
7.2 - 8.0
Osmolality:
270 - 320 mOsm/kg
Concentrated:
1 X
Product Size:
100 mL
Reagent Type:
Trypsin
Tests Performed:
In Vitro Bioassay
Shipping Condition:
Dry Ice
Regulatory Statement:
For Research Use or Further Manufacturing. Not for use in diagnostic procedures.
内容和储存
Storage conditions: -5°C to -20°C Shipping conditions: Dry ice Shelf life: 18 months from date of manufacture
引文和参考文献 (3)
Facilitated expansion of human embryonic stem cells by single-cell enzymatic dissociation.
作者
Ellerström C, Strehl R, Noaksson K, Hyllner J, Semb H
杂志
Stem Cells (2007) 25:1690-1696
产品用途
TrypLE select is better than trypsin for passage of human ES cells, which increases clone survival.
Gibco (Invitrogen) 0.25% Trypsin-EDTA was used to remove Cho-K1 cells from cell culture flasks. Gibco (Invitrogen) penicillin/streptomycin was present in the Cho-K1 cell growth medium to prevent bacterial growth. Gibco F-12K (Kaighn's modification) is optimal for growing Cho-K1 cells.
ProMMP-3 (the precursor of matrix metalloproteinase 3) and its mutant cDNA constructs were stably transfected into CHOK-1 cells using Lipofectin. Transfected cells were initially grown in glutamine-free Dulbecco's modified Eagles's medium containing dialyzed FBS and 25 µM methionine sulfoximine for 2 weeks. After colony formation, cells were split into 24-well plates and grown for additional 2 weeks in same medium with increasing concentrations of methionine sulfoximine (100-400 µM).