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- 详细信息
- 文献和实验
- 技术资料
- 库存：
2
- 供应商：
研卉生物
- 英文名：
DMEM/F-12, Powder, HEPES (Gibco®)
- 规格：
10*1L
DMEM/F-12, Powder, HEPES (Gibco®)
DMEM/F-12, Powder, HEPES
(Gibco®)
描述
Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (DMEM/F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. Life Technologies offers a variety of Gibco® DMEM/F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This DMEM/F-12 is modified as follows:
With Without
• L-glutamine • Sodium Bicarbonate
• HEPES
• Phenol Red
The complete formulation is available.
Gibco® DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids and vitamins with F-12's wide variety of components.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
cGMP Manufacturing and Quality System
Gibco® DMEM/F-12 is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001 standards. For supply chain continuity, Life Technologies offers a comparable Gibco® DMEM/F-12 product made in our Scotland facility (42400-010). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system (2.438 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
Powder forms of Gibco® cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).
详细说明
常用规格
| Glutamine: | L-Glutamine |
| Phenol Red Indicator: | Phenol Red |
| Form: | Powder |
| HEPES Buffer: | HEPES |
| Sodium Pyruvate Additive: | Sodium Pyruvate |
| Serum Supplementation: | Standard Serum Supplementation |
| Glucose: | High Glucose |
| Product Size: | 10 x 1 L |
| Shipping Condition: | Room Temperature |
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