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- 600-401-379
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 英文名：
RFP Antibody Pre-adsorbed
- 保存条件：
文献引用1000+
- 库存：
600-401-379
- 供应商：
100ul
- 规格：
100ul
红色荧光蛋白GFP抗体，RFP Antibody Pre-adsorbed
| 600-401-379 | 100ug |
600-401-379
100 µg
WB, ELISA, IHC, IF, FC, EM, FISH, IP, Multiplex, Other
RFP, mScarlet, rRFP, tdTomato
Rabbit
背景
荧光蛋白如蘑菇海葵盘状体的盘状体红色荧光蛋白（DsRed）或维多利亚水母的绿色荧光蛋白（GFP）在研究实践中被广泛使用。具有RFP和GFP的融合蛋白通常用作基因表达和蛋白质定位的标记。由于DsRed和GFP只有19%的同一性，因此，一般来说，抗GFP抗体不识别DsRed蛋白，反之亦然。在结构上，盘状体红色荧光蛋白在其总折叠（aβ-can）和发色团形成化学方面与绿锥虫绿色荧光蛋白相似。然而，盘状体红色荧光蛋白在发色团成熟过程中经历了额外的步骤，并具有专性四聚体结构。Rockland为RFP提供了许多对照、单克隆和多克隆抗体。
背景
荧光蛋白如蘑菇海葵盘状体的盘状体红色荧光蛋白（DsRed）或维多利亚水母的绿色荧光蛋白（GFP）在研究实践中被广泛使用。具有RFP和GFP的融合蛋白通常用作基因表达和蛋白质定位的标记。由于DsRed和GFP只有19%的同一性，因此，一般来说，抗GFP抗体不识别DsRed蛋白，反之亦然。在结构上，盘状体红色荧光蛋白在其总折叠（aβ-can）和发色团形成化学方面与绿锥虫绿色荧光蛋白相似。然而，盘状体红色荧光蛋白在发色团成熟过程中经历了额外的步骤，并具有专性四聚体结构。Rockland为RFP提供了许多对照、单克隆和多克隆抗体。
Product Details
Anti-RFP (RABBIT) Antibody Min X Hu Ms and Rt Serum Proteins - 600-401-379
rabbit anti-RFP antibody, DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583.
Rabbit
Polyclonal
IgG
组化使用说明：https://www.rockland.com/resources/immunohistochemistry-protocol/
RFP, mScarlet, rRFP, tdTomato
Recombinant Protein
The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full-length amino acid sequence (234aa) derived from the mushroom anemone Discosoma.
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Red Fluorescent Protein (Discosoma) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified Red Fluorescent Protein (Discosoma). No reaction was observed against Human, Mouse or Rat serum proteins.
流式使用说明：https://www.rockland.com/resources/flow-cytometry-protocol/
ELISA, IF, IHC, WB
EM, FC, FISH, IP, Other, Multiplex - View References
Polyclonal anti-RFP is designed to detect RFP and its variants. This antibody has been tested by ELISA, Western blot, IF, and IHC, and is suitable for use in EM, FC, FISH, IP, and multiplex assays based on published references. This antibody can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen. Optimal titers for applications should be determined by the researcher.
Formulation
Liquid (sterile filtered)
1.05mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
组化使用说明：https://www.rockland.com/resources/immunohistochemistry-protocol/
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文献引用1000+
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红色荧光蛋白GFP抗体，RFP Antibody Pre-adsorbed
￥3962
