- ￥3962
- rockland
- rockland
- 600-401-379
- 2025年08月29日
12 年金牌会员
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 英文名：
RFP Antibody Pre-adsorbed
- 保存条件：
文献引用1000+
- 库存：
600-401-379
- 供应商：
100ul
- 规格：
100ul
红色荧光蛋白GFP抗体，RFP Antibody Pre-adsorbed
| 600-401-379 | 100ug |
600-401-379
100 µg
WB, ELISA, IHC, IF, FC, EM, FISH, IP, Multiplex, Other
RFP, mScarlet, rRFP, tdTomato
Rabbit
背景
荧光蛋白如蘑菇海葵盘状体的盘状体红色荧光蛋白（DsRed）或维多利亚水母的绿色荧光蛋白（GFP）在研究实践中被广泛使用。具有RFP和GFP的融合蛋白通常用作基因表达和蛋白质定位的标记。由于DsRed和GFP只有19%的同一性，因此，一般来说，抗GFP抗体不识别DsRed蛋白，反之亦然。在结构上，盘状体红色荧光蛋白在其总折叠（aβ-can）和发色团形成化学方面与绿锥虫绿色荧光蛋白相似。然而，盘状体红色荧光蛋白在发色团成熟过程中经历了额外的步骤，并具有专性四聚体结构。Rockland为RFP提供了许多对照、单克隆和多克隆抗体。
背景
荧光蛋白如蘑菇海葵盘状体的盘状体红色荧光蛋白（DsRed）或维多利亚水母的绿色荧光蛋白（GFP）在研究实践中被广泛使用。具有RFP和GFP的融合蛋白通常用作基因表达和蛋白质定位的标记。由于DsRed和GFP只有19%的同一性，因此，一般来说，抗GFP抗体不识别DsRed蛋白，反之亦然。在结构上，盘状体红色荧光蛋白在其总折叠（aβ-can）和发色团形成化学方面与绿锥虫绿色荧光蛋白相似。然而，盘状体红色荧光蛋白在发色团成熟过程中经历了额外的步骤，并具有专性四聚体结构。Rockland为RFP提供了许多对照、单克隆和多克隆抗体。
Product Details
Anti-RFP (RABBIT) Antibody Min X Hu Ms and Rt Serum Proteins - 600-401-379
rabbit anti-RFP antibody, DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583.
Rabbit
Polyclonal
IgG
组化使用说明：https://www.rockland.com/resources/immunohistochemistry-protocol/
RFP, mScarlet, rRFP, tdTomato
Recombinant Protein
The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full-length amino acid sequence (234aa) derived from the mushroom anemone Discosoma.
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Red Fluorescent Protein (Discosoma) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified Red Fluorescent Protein (Discosoma). No reaction was observed against Human, Mouse or Rat serum proteins.
流式使用说明：https://www.rockland.com/resources/flow-cytometry-protocol/
ELISA, IF, IHC, WB
EM, FC, FISH, IP, Other, Multiplex - View References
Polyclonal anti-RFP is designed to detect RFP and its variants. This antibody has been tested by ELISA, Western blot, IF, and IHC, and is suitable for use in EM, FC, FISH, IP, and multiplex assays based on published references. This antibody can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen. Optimal titers for applications should be determined by the researcher.
Formulation
Liquid (sterile filtered)
1.05mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
组化使用说明：https://www.rockland.com/resources/immunohistochemistry-protocol/
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
- 作者
- 内容
- 询问日期
文献和实验该产品被引用文献
文献引用1000+
Zhang C et al. (2022). A brainstem circuit for nausea suppression. Cell Rep.
Applications
IHC, ICC, HistologyShao YQ et al. (2022). A developmental switch between electrical and neuropeptide communication in the ventromedial hypothalamus. Curr Biol.
Applications
UndefinedTwarkowski H et al. (2022). A dentate gyrusdentate gyrus-CA3 inhibitory circuit promotes evolution of hippocampal-cortical ensembles during memory consolidation. Elife.
Applications
IHC, ICC, HistologyKanada M et al. (2022). A Dual-Reporter Platform for Screening Tumor-Targeted Extracellular Vesicles. Pharmaceutics.
Applications
WB, IB, PCALeopold AV et al. (2022). A general approach for engineering RTKs optically controlled with far-red light. Nat Methods.
Applications
UndefinedCho KF et al. (2022). A light-gated transcriptional recorder for detecting cell-cell contacts. Elife.
Applications
IF, Confocal MicroscopyWang H et al. (2022). A novel spinal neuron connection for heat sensation. Neuron.
Applications
UndefinedSorrells TR et al. (2022). A persistent behavioral state enables sustained predation of humans by mosquitoes. Elife.
Applications
IF, Confocal MicroscopyBriquet M et al. (2022). Activation of lactate receptor HCAR1 down-modulates neuronal activity in rodent and human brain tissue. J Cereb Blood Flow Metab.
Applications
IHC, ICC, HistologyGodfrey N et al. (2022). Activation of LH GABAergic inputs counteracts fasting‐induced changes in tVTA/RMTG neurons. J Physiol.
Applications
UndefinedChen C et al. (2022). Agonist-Promoted Phosphorylation and Internalization of the Kappa Opioid Receptor in Mouse Brains: Lack of Connection With Conditioned Place Aversion. Front Pharmacol.
Applications
IHC, ICC, HistologyZhang KY et al. (2022). Analyses of transplanted human retinal ganglion cell morphology and localization in murine organotypic retinal explant culture. STAR Protoc.
Applications
IF, Confocal MicroscopyHenriques VJ et al. (2022). Astrocytes Modulate Somatostatin Interneuron Signaling in the Visual Cortex. Cells.
Applications
IF, Confocal Microscopy; IHC, ICC, HistologyHenriques VJ et al. (2022). Astrocytes Modulate Somatostatin Interneuron Signaling in the Visual Cortex. Cells.
Applications
IHC, ICC, HistologyYang X et al. (2022). Cerebral cavernous malformation development in chronic mouse models driven by dual recombinases induced gene deletion in brain endothelial cells. J Cereb Blood Flow Metab.
Applications
UndefinedZhou K et al. (2022). CENP-N promotes the compaction of centromeric chromatin. Nat Struct Mol Biol.
Applications
IF, Confocal MicroscopyYousefi M et al. (2022). Combinatorial Inactivation of Tumor Suppressors Efficiently Initiates Lung Adenocarcinoma with Therapeutic Vulnerabilities. Cancer Res.
Applications
IHC, ICC, HistologyYoshinaga S et al. (2022). Dab1-deficient deep layer neurons prevent Dab1-deficient superficial layer neurons from entering the cortical plate. Neurosci Res.
Applications
UndefinedQu R et al. (2022). Decomposing a deterministic path to mesenchymal niche formation by two intersecting morphogen gradients. Dev Cell.
Applications
UndefinedAdemi H et al. (2022). Deciphering the origins and fates of steroidogenic lineages in the mouse testis. Cell Rep.
Applications
IF, Confocal MicroscopyLiu Z et al. (2022). Deficiency in endocannabinoid synthase DAGLB contributes to early onset Parkinsonism and murine nigral dopaminergic neuron dysfunction. Nat Commun.
Applications
IHC, ICC, HistologyCanton-Josh JE et al. (2022). Dopaminergic regulation of vestibulo-cerebellar circuits through unipolar brush cells. Elife.
Applications
IHC, ICC, HistologyWang H et al. (2022). Dual Cre and Dre recombinases mediate synchronized lineage tracing and cell subset ablation in vivo. J Biol Chem.
Applications
IF, Confocal MicroscopyPark HH et al. (2022). Dual-functional hydrogel system for spinal cord regeneration with sustained release of arylsulfatase B alleviates fibrotic microenvironment and promotes axonal regeneration. Biomaterials.
Applications
IHC, ICC, HistologyEroglu E et al. (2022). Epicardium-derived cells organize through tight junctions to replenish cardiac muscle in salamanders. Nat Cell Biol.
Applications
IF, Confocal Microscopy相关实验
诺奖得主 Jennifer 最新研究：CRISPR/Cas12c 无需切割 DNA 就可实现基因沉默，抵御病毒入侵
-crRNA 的处理，如果没有 pre-crRNA 的处理，Cas12c 则无法有效地结合目标 dsDNA。 Cas12c 可以在不触发 DNase 活性的情况下阻断基因表达 前期的研究发现，Cas12c 可以靶向细菌中生存必需的基因，这可能是由于体内 Cas12c 介导的基因组靶点的切割，也可能是 Cas12c 通过靶向聚合酶阻断反应来介导转录沉默。为了探索这些可能性，研究人员利用大肠杆菌开发了一种双色荧光干扰试验，其中编码红色荧光蛋白（RFP）或绿色荧光蛋白（GFP）的报告基因被整合到大肠杆菌基因
IMMUNOSCREENING OF WITH ANTIBODY PROBES
in 10mM IPTG (MW=238.31). Briefly blot the excess solution from the filters, and wrap them in cellophane. These should be moist and stored at 4°C. We typically adsorb serum with five 150 mm Petri dishes of lysed E. coli to reduce anti-E. coli antibody
How Do I make an Antibody against a Peptides
If your peptide is synthesized with a terminal cysteine residue (i.e., a free sulfhydryl group, -SH), you can use any maleimide-activated carrier protein to create an immunogen. One-step chemical reactions are easy with Pierce Sulfo-SMCC Pre
技术资料暂无技术资料 索取技术资料
文献支持
红色荧光蛋白GFP抗体，RFP Antibody Pre-adsorbed
￥3962
