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文献和实验Large Scale Plasmid Preps: Qiagen/Cesium Method
most of it will pellet along with the bacterial cell wall. However the relative purity of the plasmid DNA will drop if the plasmid is large or at a relatively low copy number in the prep. The combination of a kit prep with cesium banding eliminates this problem
Heat at 94°C for 10 min. Precipitate the DNA using 0.1 x volume 3 M sodium acetate, and 2.5 x volume 100% ethanol. Resuspend the DNA pellet in 40 µl water. To 12.2 µl DNA from #10 above, add the following re-amplification
Large Scale Preps: (See Large scale plsasmid prep protocol for more details) Cultures: Inoculate a 5 mL LB/Amp (50 - 100 µg/mL) culture in early a.m. with a single colony. Use all 5 mL to inoculate a 500 mL LB/Amp culture
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