INNER BUCKET PIR 4.1 (SET 2)

Chromatin Immunoprecipitation (ChIP) on Unfixed Chromatin from Cells and Tissues to Analyze Histone Modifications

Washing buffer A Washing buffer B Washing buffer C Equipment Centrifuges:   Bench-top centrifuge with cooling system for 1.5-ml microcentrifuge tubes   Centrifuge with a swing-out bucket rotor for 15-ml

Size and Shape of Protein Molecules at the Nanometer Level Determined by Sedimentation, Gel Filtration, and Electron Microscopy

gradients have been most frequently used, but we prefer 15�40% glycerol gradients in 0.2 M ammonium bicarbonate, because this is the buffer used for rotary shadowing EM (Section   6 ). The protein of interest is sedimented in one bucket of the swinging