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文献和实验E L I S A w i t h p l a t e l e t s
prepare platelets from control and immunized mice distribute 2.5 x106 platelets per well on F-bottomed MaxiSorp Nunc-Immuno microplates (GibcoBRL, Roskilde, Denmark), centrifuge at 3000 rpm, 7 min, 10ѓC. fix with PBS-PF
DNAs per Biomek 1000 robotic workstation within 3 hours (26). Basically, M13 plaques are picked with sterile toothpicks into aliquots of early log phase JM101, prepared as discussed above. The phage infected cultures are incubated in a 37degC shaker
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
for 1.5-mL tubes (Invitrogen) Microcentrifuge, refrigerated Microscope, inverted Pasteur pipettes Pipettors and tips, 5- to 1000-µL Refrigerator or cold room preset to 4°C Rotating wheel/platform Shaking incubator preset
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