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文献和实验Preparation of DNA Template For Direct Sequencing of Large Insert PAC and BAC Plasmid
each block with an AirPore™ (Qiagen Inc.) sheet and incubate at 37?C with shaking at 325 RPM for approximately 20 hours. 2. Pellet cells by centrifugation for 20’ at 3,200 RPM in a Sorvall RT7 at 4?C with RTH-250 rotor, ~1700 xg. Decant and drain pellets
Sucrose Fractionation of Castor Bean
gradients; one for a linear 33-60% gradient and the other for a stepped gradient. The volume of each solution will depend on the centrifuge rotor to be used and its corresponding centrifuge tube capacity. The following are directions for 35 ml centrifuge
Alkaline Lysis and CsCl Purification of BAC DNA for Transgenic Production.
of overnight culture to inoculate 500ml of LB (+antibiotic) x 2 for a total culture volume of 1L, shake overnight (about 16hrs) at 37°C. 3. Collect cells by centrifugation in 250ml x 4 Nalgene bottles, (H-6000A rotor, 3800 rpm), 10 minutes, 4°C.
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