GenomeLab™ Human STR Primer Se

Constructing STR Multiplex Assays

have become widely used in forensic DNA typing. This chapter will focus on some of the aspects of constructing robust STR multiplex assays, including careful design and quality control of PCR primers. Examples from the development of a cat STR 12plex and a human Y

PCR引物设计和优化(PCR PRIMER DESIGN AND REACTION OPTIMISATION)

of this is nested primer PCR: PCR amplification is performed with one set of primers, then some product is taken - with or without removal of reagents - for re-amplification with an internally-situated, “nested” set of primers.  This process adds another level

Biotinylated Primer for Detecting Telomerase Activity Without Amplification

The activation of telomerase, proposed as a critical set for cell immortalization to overcome cellular senescence, was believed to be associated with the malignant progression of human tumours (1 -4 ). Therefore human telomerase appears