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文献和实验Transformation of E. coli by Electroporation
100% ethanol 70% ethanol 0.5X TE SOC medium transformation plates I. Preparation of E. coli cells
Cetyltrimethylammonium bromide (CTAB) Plant DNA Extraction
, mix, and place on ice for 10 min. 7. Centrifuge at 1,000 X g for 5 min to collect the precipitate. 8. Discard the supernatant and dry the inside of the tube with a paper towel (do not dry the pellet). 9. Add 4 ml of TE Buffer
or cosmid DNA of interest into a 12 X 75 mm Falcon tube containing 2 ml of LB media supplemented with the appropriate antibiotic (typically ampicillin at 100 ug/ml) and incubate at 37deg C 8-10 hours with shaking at 250 rpm. Transfer the culture
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