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文献和实验or cosmid DNA of interest into a 12 X 75 mm Falcon tube containing 2 ml of LB media supplemented with the appropriate antibiotic (typically ampicillin at 100 ug/ml) and incubate at 37deg C 8-10 hours with shaking at 250 rpm. Transfer the culture
at lysine 4 of H3 (H3K4Me2; Upstate Ltd.). Radioactive PCR was performed on bound (B) and unbound (U) fractions with primers that amplified from a unique sequence at an imprinting-control center located in a gene called Kvlqt1. The PCR products
RNase Protection Assay (Beverly Faulkner-Jones)
and a yellow b-counter, for a low abundance mRNA, 100 - 200 cps / ml is sufficient and for a high abundance mRNA 500 - 600 cps / ml is need 9 Vortex tube and then heat denature the reactions at 80oC for 2-5 minutes and transfer to a pre-warmed rack at 45oC
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