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文献和实验Monoclonal antibodies (mAbs) specific for cell surface antigens are an invaluable tool to study immune receptor expression and function. Here, we outline a generalized reporter cell-based approach to the generation and high-throughput
; they are said to demonstrate contact inhibition.Consequently, growth curves are not measured by removing aliquots and determining cell concentration, but are measured by the density of cells growing on the vessel surface. This is accomplished through the use
Microsphere Surface Protein Determination Using Flow Cytometry
an extrinsic staining protocol using the amine?reactive CBQCA dye to measure the amount of protein on the surface of a microsphere. This approach is novel in that it allows microspheres bearing proteins without known binding partners to be accurately quantified
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