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文献和实验Using the Wash U Epigenome Browser to Examine Genome‐Wide Sequencing Data
Abstract Table of Contents Figures Literature Cited Abstract This unit describes the Wash U
(1992) The oligonucleotides we use are kits, contaning 20 decamers, from Operon Technologies Inc. . I. DNase I treatment of RNA mix and incubate for 30 min. at 37 °C : 50 µgRNA, 10 µl RNase inhibitor (1 U/µl), 1 µl
Two-hybrid analysis of genetic
and prey strains (see Protocol 1) One set of the following 150 x 15 mm plates for each test of interactions between an activation domain-tagged protein (in a prey strain)and 96 baits (bait strains): -u-h- Glu; -w Glu; YPD; -u-h-w Glu X-Gal; -u-h-w Gal/Raf
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