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文献和实验Hybridization of High Density Arrayed BAC Nylon Filter Blots
per sample well. Mixture (each sample) 2.5µl 10X reaction buffer (Perkin Elmer) 2.5µl MgCl2 (25mM) 2.0µl dNTPs (2.5mM) 1.0µl vector primer 1 (5µM) 1.0µl vector primer 2 (5µM) 0.5µl AmpliTaq (Perkin Elmer) 14.5µl ddH2 O Run
Two dimensional peptide mapping
Transfer of the protein to a filter is much superior to homogenization of the prep gel and elution. Although some people have concern about inefficient transfer of large proteins and loss of hydrophobic peptides on the filters, we have had good success
Phosphoamino acid analysis:Mark Kamps's method
your sample.We use 0.1 mm EM cellulose plates You can spot the whole sample if you are skillful and have no choice because you don't have many counts. Spot 0.25-0.30 µl at a time and dry with house air,blown through a plugged pipet,between applications
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