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文献和实验behind acrylic shield. Syringe needle will be stored/discarded in capped centrifuge tube behind acrylic shield and discarded in solid waste. 16. While probe is boiling, place 32P in acrylic lock box and return box and labeling kit reagents to freezer
µl 10 mM hydroquinone 110 µl DNA (+ NaOH) 10 µl water 5.Gently mix and overlay with mineral oil. Cover the tube with aluminum foil to shield from the light. 6.Incubate at 55℃ for 16-18 h. 7.Purify DNA using the Geneclean II kit
Cloning via Restriction Digests
purification kit or QIAquick Gel Extraction kit). Prepare agarose gel. Low melting point agarose will facilitate DNA extraction at a later step, but regular agarose also works. To make a 0.8% agarose gel, use 0.8 g
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