Drive Kit,Optima MAX-XP

MicroElute Gel Extraction Kit

of 10-15 ug DNA. If the expected yield is larger, divide the sample into the appropriate number of columns.    5) Add 300 ul of Binding Buffer (XP2) into the column. Centrifuge at 10,000 x g for 1 min at room temperature to wash the column. Discard

Using Soil DNA Kit (D5625) to Clean Up DNA containing inhibitors

) and incubate for 4 minutes. Centrifuge at max speed for 30 seconds and discard flowthrough. 7. Add equal volume of XP2 buffer to the cleared lysate, mix the sample throughly by vortexing. 8. Apply entire sample including any precipitation

DNA聚合酶，你选对了吗？

验对保真度要求较高，在选择 DNA 聚合酶的时候就需要使用高保真酶，如 Pfu、KOD、Primerstar、Phanta 等。前三者都属于第一代高保真酶，已在市场中广泛使用。诺唯赞科研团队的大力改造生产的 Phanta 系列的高保真酶，保真度达到了 Taq 酶的 50 倍，扩增性能、扩增长度、产量、模板适应性都显著提升。Phanta Max 保真度是 Taq 酶的 53 倍，Pfu 的 6 倍，对于质粒等简单模板有效扩增长度可达 40kb，cDNA 有效扩增长度可达 10kb，基因组有效扩增