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文献和实验三句话读懂一篇 CNS:线粒体突变降低癌症死亡风险 57% 以上;解密大猩猩捶胸口之谜...
partitioning in the tumour microenvironment. Nature (2021). https://doi.org/10.1038/s41586-021-03442-13. Gorelick, A.N., Kim, M., Chatila, W.K. et al. Respiratory complex and tissue lineage drive recurrent mutations in tumour mtDNA. Nat Metab (2021). https
【精华】vol 687 Chapter 3 采用Splinkerette-PCR技术对前病毒基因组插入位点进行分离
to the digests to drive the primer extension reaction. Pfu DNA polymerase is used to produce blunt-end doublestrandedDNA fragments. The extension products are subsequentlypurified with streptavidin magnetic particles, prior to ligation toSplinkBlunt
miRNA microarrays(McManus Lab)(芯片)
Diamond-tipped glass etching pen (VWR 52865-005) StrataLinker for UV cross-linking Protocol a) Pour 100 ml 0.5x SSC into hydration tray and warm on slide warmer. Set the warmer to 37℃ b) Pre-heat a heating block at max (>100
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